Improvement of an antibody-enzyme coupling yield by enzyme surface supercharging
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چکیده
منابع مشابه
Improvement of an antibody-enzyme coupling yield by enzyme surface supercharging
BACKGROUND Protein cross-coupling reactions demand high yields, especially if the products are intended for bioanalytics, like enzyme-linked immunosorbent assays. Amongst other factors, the coupling yield depends on the concentration of the proteins being used for coupling. Protein supercharging of enzymes can increase the solubility dramatically, which could promote enzyme-antibody coupling re...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2014
ISSN: 1472-6750
DOI: 10.1186/s12896-014-0088-6